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horse serum proteins  (Jackson Immuno)


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    Jackson Immuno horse serum proteins
    Horse Serum Proteins, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/horse+serum+proteins/Peroxidase+AffiniPure+F(ab')%E2%82%82+Fragment+Goat+Anti-Mouse+IgG%2C+Fc%CE%B3+fragment+specific/pm41895286-1043-19-23
    Average 93 stars, based on 48 article reviews
    horse serum proteins - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Comparison of Skin Prick Tests (SPT), Intradermal Tests (IDT) and In Vitro Tests in the Characterization of Insect Bite Hypersensitivity (IBH) in a Population of Lusitano Horses: Contribution for Future Implementation of SPT in IBH Diagnosis
    Article Snippet: .. After a further washing step, an alkaline-phosphatase-conjugated goat anti mouse IgG with minimal cross reactivity to horse serum proteins (Jackson Immuno Research, West Grove, PA, USA) was added and incubated for 1.5 h at RT on a shaker. .. After final washes, plates were developed with 1.5 mg/mL phosphatase substrate (Sigma, St. Louis, MO, USA) in 10% diethanolamine (Fluka; Sigma-Aldrich, St-Louis.

    Article Title: Comparison of Skin Prick Tests (SPT), Intradermal Tests (IDT) and In Vitro Tests in the Characterization of Insect Bite Hypersensitivity (IBH) in a Population of Lusitano Horses: Contribution for Future Implementation of SPT in IBH Diagnosis.
    Article Snippet: .. After a further washing step, an alkaline-phosphatase-conjugated goat anti mouse IgG with minimal cross reactivity to horse serum proteins (Jackson Immuno Research, West Grove, PA, USA) was added and incubated for 1.5 h at RT on a shaker. .. After final washes, plates were developed with 1.5 mg/mL phosphatase substrate (Sigma, St. Louis, MO, USA) in 10% diethanolamine (Fluka; Sigma-Aldrich, St-Louis.

    Expressing:

    Article Title: Lipid nanoparticles outperform electroporation in mRNA-based CAR T cell engineering
    Article Snippet: .. CAR expression, viability, and surface markers of the transfected T cells were flow cytometrically analyzed on BD FACSCanto II (BD Biosciences, Mississauga, Canada) using AlexaFluor 647-conjugated AffiniPure F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal cross-reactions to human, bovine, and horse serum proteins (referred to as anti-F(ab’) 2 –Alexa Fluor 647 reagent; Jackson ImmunoResearch, Ely, UK), 7-aminoactinomycin D (7-AAD; BD Biosciences), FITC Annexin V (BD Biosciences), PE-Cy5 CD3 (clone: UCHT-1, BD Biosciences), BV510 CD4 (clone: SK3, BD Biosciences), PE-Cy7 CD8 (clone: RPA-T8, BD Biosciences), PE CD25 (clone: 2A3, BD Biosciences), BV421 LAG-3 (clone: T47-530, BD Biosciences), and PE-Cy7 LAG-3 (clone: 3DS223H, Invitrogen by ThermoFisher Scientific). .. The data were evaluated in Kaluza Analysis software (version 2.1; Beckman Coulter, Krefeld, Germany).

    Article Title: Optimal Chimeric Antigen Receptor (CAR)-mRNA for Transient CAR T Cell Generation.
    Article Snippet: 4.5.2. mRNA-LNP Formulation and T Cell Transfection As an alternative mRNA delivery method, mRNA-LNPs were formulated using the GenVoy-ILM T Cell Kit for mRNA (#1000701, Cytiva, now including Precision NanoSystems, Marlborough, MA, USA), and incubated with T cells as previously described by us in detail [46]. .. CAR expression of the transfected T cells was flow cytometrically analyzed on BD FACSCantoTM II (BD Biosciences, Mississauga, ON, Canada) or on MACSQuant10 (Miltenyi Biotec, Bergisch Gladbach, Germany) using PE or AlexaFluor 647-conjugated AffiniPure polyclonal goat F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal crossreactions to human, bovine, and horse serum proteins (#JIM-005-600-006, Jackson ImmunoResearch, Ely, UK) or APC-coupled anti-DYKDDDDK (FLAG) Tag Antibody (clone: L5) (#637307, BioLegend, San Diego, CA, USA). .. Ten minutes prior to measurement, 7- aminoactinomycin D (7-AAD, #559925, BD Biosciences, Mississauga, Canada) was added to stain dead cells.

    Article Title: Lipid nanoparticles outperform electroporation in mRNA-based CAR T cell engineering
    Article Snippet: .. Flow cytometry CAR expression, viability, and surface markers of the transfected T cells were flow cytometrically analyzed on BD FACSCanto II (BD Biosciences, Mississauga, Canada) using AlexaFluor 647-conjugated AffiniPure F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal cross-reactions to human, bovine, and horse serum proteins (referred to as anti-F(ab’) 2 –Alexa Fluor 647 reagent; Jackson ImmunoResearch, Ely, UK), 7-aminoactinomycin D (7-AAD; BD Biosciences), FITC Annexin V (BD Biosciences), PE-Cy5 CD3 (clone: UCHT-1, BD Biosciences), BV510 CD4 (clone: SK3, BD Biosciences), PE-Cy7 CD8 (clone: RPA-T8, BD Biosciences), PE CD25 (clone: 2A3, BD Biosciences), BV421 LAG-3 (clone: T47-530, BD Biosciences), and PE-Cy7 LAG-3 (clone: 3DS223H, Invitrogen by ThermoFisher Scientific). .. The data were evaluated in Kaluza Analysis software (version 2.1; Beckman Coulter, Krefeld, Germany). mRNA quantification inside the cells At each analysis time point, cells were harvested and washed in PBS.

    Article Title: Optimal Chimeric Antigen Receptor (CAR)-mRNA for Transient CAR T Cell Generation
    Article Snippet: As an alternative mRNA delivery method, mRNA-LNPs were formulated using the GenVoy-ILM T Cell Kit for mRNA (#1000701, Cytiva, now including Precision NanoSystems, Marlborough, MA, USA), and incubated with T cells as previously described by us in detail [ ]. .. CAR expression of the transfected T cells was flow cytometrically analyzed on BD FACSCanto TM II (BD Biosciences, Mississauga, ON, Canada) or on MACSQuant10 (Miltenyi Biotec, Bergisch Gladbach, Germany) using PE or AlexaFluor 647-conjugated AffiniPure polyclonal goat F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal cross-reactions to human, bovine, and horse serum proteins (#JIM-005-600-006, Jackson ImmunoResearch, Ely, UK) or APC-coupled anti-DYKDDDDK (FLAG) Tag Antibody (clone: L5) (#637307, BioLegend, San Diego, CA, USA). .. Ten minutes prior to measurement, 7-aminoactinomycin D (7-AAD, #559925, BD Biosciences, Mississauga, Canada) was added to stain dead cells.

    Transfection:

    Article Title: Lipid nanoparticles outperform electroporation in mRNA-based CAR T cell engineering
    Article Snippet: .. CAR expression, viability, and surface markers of the transfected T cells were flow cytometrically analyzed on BD FACSCanto II (BD Biosciences, Mississauga, Canada) using AlexaFluor 647-conjugated AffiniPure F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal cross-reactions to human, bovine, and horse serum proteins (referred to as anti-F(ab’) 2 –Alexa Fluor 647 reagent; Jackson ImmunoResearch, Ely, UK), 7-aminoactinomycin D (7-AAD; BD Biosciences), FITC Annexin V (BD Biosciences), PE-Cy5 CD3 (clone: UCHT-1, BD Biosciences), BV510 CD4 (clone: SK3, BD Biosciences), PE-Cy7 CD8 (clone: RPA-T8, BD Biosciences), PE CD25 (clone: 2A3, BD Biosciences), BV421 LAG-3 (clone: T47-530, BD Biosciences), and PE-Cy7 LAG-3 (clone: 3DS223H, Invitrogen by ThermoFisher Scientific). .. The data were evaluated in Kaluza Analysis software (version 2.1; Beckman Coulter, Krefeld, Germany).

    Article Title: Optimal Chimeric Antigen Receptor (CAR)-mRNA for Transient CAR T Cell Generation.
    Article Snippet: 4.5.2. mRNA-LNP Formulation and T Cell Transfection As an alternative mRNA delivery method, mRNA-LNPs were formulated using the GenVoy-ILM T Cell Kit for mRNA (#1000701, Cytiva, now including Precision NanoSystems, Marlborough, MA, USA), and incubated with T cells as previously described by us in detail [46]. .. CAR expression of the transfected T cells was flow cytometrically analyzed on BD FACSCantoTM II (BD Biosciences, Mississauga, ON, Canada) or on MACSQuant10 (Miltenyi Biotec, Bergisch Gladbach, Germany) using PE or AlexaFluor 647-conjugated AffiniPure polyclonal goat F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal crossreactions to human, bovine, and horse serum proteins (#JIM-005-600-006, Jackson ImmunoResearch, Ely, UK) or APC-coupled anti-DYKDDDDK (FLAG) Tag Antibody (clone: L5) (#637307, BioLegend, San Diego, CA, USA). .. Ten minutes prior to measurement, 7- aminoactinomycin D (7-AAD, #559925, BD Biosciences, Mississauga, Canada) was added to stain dead cells.

    Article Title: Lipid nanoparticles outperform electroporation in mRNA-based CAR T cell engineering
    Article Snippet: .. Flow cytometry CAR expression, viability, and surface markers of the transfected T cells were flow cytometrically analyzed on BD FACSCanto II (BD Biosciences, Mississauga, Canada) using AlexaFluor 647-conjugated AffiniPure F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal cross-reactions to human, bovine, and horse serum proteins (referred to as anti-F(ab’) 2 –Alexa Fluor 647 reagent; Jackson ImmunoResearch, Ely, UK), 7-aminoactinomycin D (7-AAD; BD Biosciences), FITC Annexin V (BD Biosciences), PE-Cy5 CD3 (clone: UCHT-1, BD Biosciences), BV510 CD4 (clone: SK3, BD Biosciences), PE-Cy7 CD8 (clone: RPA-T8, BD Biosciences), PE CD25 (clone: 2A3, BD Biosciences), BV421 LAG-3 (clone: T47-530, BD Biosciences), and PE-Cy7 LAG-3 (clone: 3DS223H, Invitrogen by ThermoFisher Scientific). .. The data were evaluated in Kaluza Analysis software (version 2.1; Beckman Coulter, Krefeld, Germany). mRNA quantification inside the cells At each analysis time point, cells were harvested and washed in PBS.

    Article Title: Optimal Chimeric Antigen Receptor (CAR)-mRNA for Transient CAR T Cell Generation
    Article Snippet: As an alternative mRNA delivery method, mRNA-LNPs were formulated using the GenVoy-ILM T Cell Kit for mRNA (#1000701, Cytiva, now including Precision NanoSystems, Marlborough, MA, USA), and incubated with T cells as previously described by us in detail [ ]. .. CAR expression of the transfected T cells was flow cytometrically analyzed on BD FACSCanto TM II (BD Biosciences, Mississauga, ON, Canada) or on MACSQuant10 (Miltenyi Biotec, Bergisch Gladbach, Germany) using PE or AlexaFluor 647-conjugated AffiniPure polyclonal goat F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal cross-reactions to human, bovine, and horse serum proteins (#JIM-005-600-006, Jackson ImmunoResearch, Ely, UK) or APC-coupled anti-DYKDDDDK (FLAG) Tag Antibody (clone: L5) (#637307, BioLegend, San Diego, CA, USA). .. Ten minutes prior to measurement, 7-aminoactinomycin D (7-AAD, #559925, BD Biosciences, Mississauga, Canada) was added to stain dead cells.

    Recombinase Polymerase Amplification:

    Article Title: Lipid nanoparticles outperform electroporation in mRNA-based CAR T cell engineering
    Article Snippet: .. CAR expression, viability, and surface markers of the transfected T cells were flow cytometrically analyzed on BD FACSCanto II (BD Biosciences, Mississauga, Canada) using AlexaFluor 647-conjugated AffiniPure F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal cross-reactions to human, bovine, and horse serum proteins (referred to as anti-F(ab’) 2 –Alexa Fluor 647 reagent; Jackson ImmunoResearch, Ely, UK), 7-aminoactinomycin D (7-AAD; BD Biosciences), FITC Annexin V (BD Biosciences), PE-Cy5 CD3 (clone: UCHT-1, BD Biosciences), BV510 CD4 (clone: SK3, BD Biosciences), PE-Cy7 CD8 (clone: RPA-T8, BD Biosciences), PE CD25 (clone: 2A3, BD Biosciences), BV421 LAG-3 (clone: T47-530, BD Biosciences), and PE-Cy7 LAG-3 (clone: 3DS223H, Invitrogen by ThermoFisher Scientific). .. The data were evaluated in Kaluza Analysis software (version 2.1; Beckman Coulter, Krefeld, Germany).

    Article Title: Lipid nanoparticles outperform electroporation in mRNA-based CAR T cell engineering
    Article Snippet: .. Flow cytometry CAR expression, viability, and surface markers of the transfected T cells were flow cytometrically analyzed on BD FACSCanto II (BD Biosciences, Mississauga, Canada) using AlexaFluor 647-conjugated AffiniPure F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal cross-reactions to human, bovine, and horse serum proteins (referred to as anti-F(ab’) 2 –Alexa Fluor 647 reagent; Jackson ImmunoResearch, Ely, UK), 7-aminoactinomycin D (7-AAD; BD Biosciences), FITC Annexin V (BD Biosciences), PE-Cy5 CD3 (clone: UCHT-1, BD Biosciences), BV510 CD4 (clone: SK3, BD Biosciences), PE-Cy7 CD8 (clone: RPA-T8, BD Biosciences), PE CD25 (clone: 2A3, BD Biosciences), BV421 LAG-3 (clone: T47-530, BD Biosciences), and PE-Cy7 LAG-3 (clone: 3DS223H, Invitrogen by ThermoFisher Scientific). .. The data were evaluated in Kaluza Analysis software (version 2.1; Beckman Coulter, Krefeld, Germany). mRNA quantification inside the cells At each analysis time point, cells were harvested and washed in PBS.

    FLAG-tag:

    Article Title: Optimal Chimeric Antigen Receptor (CAR)-mRNA for Transient CAR T Cell Generation.
    Article Snippet: 4.5.2. mRNA-LNP Formulation and T Cell Transfection As an alternative mRNA delivery method, mRNA-LNPs were formulated using the GenVoy-ILM T Cell Kit for mRNA (#1000701, Cytiva, now including Precision NanoSystems, Marlborough, MA, USA), and incubated with T cells as previously described by us in detail [46]. .. CAR expression of the transfected T cells was flow cytometrically analyzed on BD FACSCantoTM II (BD Biosciences, Mississauga, ON, Canada) or on MACSQuant10 (Miltenyi Biotec, Bergisch Gladbach, Germany) using PE or AlexaFluor 647-conjugated AffiniPure polyclonal goat F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal crossreactions to human, bovine, and horse serum proteins (#JIM-005-600-006, Jackson ImmunoResearch, Ely, UK) or APC-coupled anti-DYKDDDDK (FLAG) Tag Antibody (clone: L5) (#637307, BioLegend, San Diego, CA, USA). .. Ten minutes prior to measurement, 7- aminoactinomycin D (7-AAD, #559925, BD Biosciences, Mississauga, Canada) was added to stain dead cells.

    Article Title: Optimal Chimeric Antigen Receptor (CAR)-mRNA for Transient CAR T Cell Generation
    Article Snippet: As an alternative mRNA delivery method, mRNA-LNPs were formulated using the GenVoy-ILM T Cell Kit for mRNA (#1000701, Cytiva, now including Precision NanoSystems, Marlborough, MA, USA), and incubated with T cells as previously described by us in detail [ ]. .. CAR expression of the transfected T cells was flow cytometrically analyzed on BD FACSCanto TM II (BD Biosciences, Mississauga, ON, Canada) or on MACSQuant10 (Miltenyi Biotec, Bergisch Gladbach, Germany) using PE or AlexaFluor 647-conjugated AffiniPure polyclonal goat F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal cross-reactions to human, bovine, and horse serum proteins (#JIM-005-600-006, Jackson ImmunoResearch, Ely, UK) or APC-coupled anti-DYKDDDDK (FLAG) Tag Antibody (clone: L5) (#637307, BioLegend, San Diego, CA, USA). .. Ten minutes prior to measurement, 7-aminoactinomycin D (7-AAD, #559925, BD Biosciences, Mississauga, Canada) was added to stain dead cells.

    Flow Cytometry:

    Article Title: Lipid nanoparticles outperform electroporation in mRNA-based CAR T cell engineering
    Article Snippet: .. Flow cytometry CAR expression, viability, and surface markers of the transfected T cells were flow cytometrically analyzed on BD FACSCanto II (BD Biosciences, Mississauga, Canada) using AlexaFluor 647-conjugated AffiniPure F(ab’)2 Anti-Mouse IgG, F(ab’)2 fragment specific with minimal cross-reactions to human, bovine, and horse serum proteins (referred to as anti-F(ab’) 2 –Alexa Fluor 647 reagent; Jackson ImmunoResearch, Ely, UK), 7-aminoactinomycin D (7-AAD; BD Biosciences), FITC Annexin V (BD Biosciences), PE-Cy5 CD3 (clone: UCHT-1, BD Biosciences), BV510 CD4 (clone: SK3, BD Biosciences), PE-Cy7 CD8 (clone: RPA-T8, BD Biosciences), PE CD25 (clone: 2A3, BD Biosciences), BV421 LAG-3 (clone: T47-530, BD Biosciences), and PE-Cy7 LAG-3 (clone: 3DS223H, Invitrogen by ThermoFisher Scientific). .. The data were evaluated in Kaluza Analysis software (version 2.1; Beckman Coulter, Krefeld, Germany). mRNA quantification inside the cells At each analysis time point, cells were harvested and washed in PBS.

    other:

    Article Title: Analysis of GRK2 aggregation in the pathology of Alzheimer disease in animal models.
    Article Snippet: Secondary antibodies: Peroxidase-conjugated AffiniPure F(ab’) 2 Fragment Goat Anti-Mouse IgG, Fcgamma Fragment Specific (minimal cross-reaction to Human, Bovine, and Horse Serum Proteins) (115-036-071; Jackson ImmunoResearch Laboratories; Lot 77832; Lot 118230; Lot 151768); Peroxidase-conjugated AffiniPure F(ab’) 2 Fragment Goat Anti-Rabbit IgG, Fcgamma Fragment Specific (minimal cross-rection to Human Serum Proteins) (111-036-046; Jackson ImmunoResearch Laboratories; Lot 84989; Lot 113992); Protein A, Peroxidase Conjugate, Native Protein A from Staphylococcus aureus conjugated to horseradish peroxidase (Cat. No. 539253; Calbiochem; Lot 3815982); Protein G, HRP conjugate (Catalog #18–161; Upstate/CHEMICON/Linco, EMD Millipore Corporation; Lot # 3734880); Alexa Fluor 488 goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody (A-11029; Invitrogen Molecular Probes; Lot 57465A); Alexa Fluor 568 goat anti-Rabbit IgG (H + L) (A-11011; Invitrogen by Thermo Fisher Scientific; Lot 1811756).



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